To further test this notion, rats were subjected to cerebral ischemia, and their brains were collected to measure HIF-1α activity by ELISA (Fig 4A), and STI-1 expression by immunofluorescence (Fig 4B), Western blot (Fig 4C) and immunohistochemistry (Fig 4D). The gene discussed is HIF1A; the disease is Cerebral ischemia.