PTEN and gastric cancer: To investigate whether miR-214 directly can alter the expression of PTEN in gastric cancer cells, a fragment of the 3’-UTR of PTEN mRNA, containing the putative miR-214 binding sequence, was cloned into a firefly luciferase reporter construct, and cotransfected with a control Renilla luciferase reporter construct into gastric cancer cells along with either anti-miR-214 or control anti-miRNA.