Injury in metabolic liver disease and NASH, which is strongly correlated with impaired hepatic insulin signaling, has been attributed to the recruitment of proinflammatory, M1-polarized macrophages.24 To address whether STZ-induced hypoinsulinemia affects intrahepatic immune cell populations, FACS analysis of CD45+ liver cells was performed. This evidence concerns the gene PTPRC and metabolic dysfunction-associated steatohepatitis.