To investigate whether retinal resident microglia proliferation accounts for the significantly increased number of AF spots observed in rd7/rd7;Tg/Tg mice injected with AP20187, we performed EdU labeling and Iba-1 immunostaining in rd7/rd7;Tg/Tg mice 1 month after treatment with AP20187 or vehicle. This evidence concerns the gene NR2E3 and atrial fibrillation.