Here we verify in four distinct primary pancreatic cancer cell lines derived from patient tumors that cancer stem cell-enriched sphere cultures indeed show marked overexpression of SHH and the Hedgehog target genes GLI-1 and GLI-2 (Fig. 1A), as well as increased mTOR pathway activity (Fig. 1B). The gene discussed is GLI1; the disease is familial pancreatic carcinoma.