Under phase contrast microscopy, OBs were seen in the cells transfected individually with vAcPH-gfp, vAce25ko-rep-PH-gfp, vAcPie1-e25-PH-gfp and vAce25ko-PH-gfp at late times, but few OB-containing cells and no evidence of the spreading of the infection was observed in the transfections with vAcPie1-e25-PH-gfp or vAce25ko-PH-gfp (data not shown). The gene discussed is ITM2C; the disease is infection.