Lack of Lmo3 expression was confirmed in (1) WAT of a mouse tissue library (Figure S2E), (2) the murine 3T3-L1 adipocyte cell model throughout differentiation (Figures 2J and S2F), and (3) differentiating murine primary preadipocytes (Figure 2K), as well as in (4) ASCs and mature adipocytes isolated from chow-fed or high-fat diet (HFD)-challenged mice (Figures 2L and S2G), (5) distinct SC and VI WAT depots and BAT of mice fed a low-fat diet (LFD) or HFD (Figures 2M and S2H), and (6) WAT obtained from a genetic obesity mouse model (db/db) on both LFD and HFD (Figures 2N and S2I). This evidence concerns the gene LMO3 and obesity due to melanocortin 4 receptor deficiency.