As far as TNF-α was concerned, statistically significant difference between groups was identified (PAD group = 1.14 ± 0.45; non-PAD group = 1.04 ± 0.45; t-test: statistics = 2.105, P-value = 0.0360), but this difference has no clinical significance since the values in both groups are within normal range (0.550–2.816 pg/mL). Here, TNF is linked to peripheral arterial disease.