Previous data have shown that Mtm1 mice and mtm1 zebrafish morphants display an increase in PtdIns3P levels (the substrate of myotubularin) [11], [13] To investigate if mtmr12 knockdown also affects PItdIns3P levels in skeletal muscle as seen in myotubular myopathy, PtdIns3P staining was performed on control and mtmr12 morphant skeletal muscle. This evidence concerns the gene MTM1 and centronuclear myopathy.