We selected 5 CpG loci with significant methylation differences by tumor histology (q-value < 2.2E-16 and fold-change > 2.50) for validation by Pyrosequencing (HOXA9_E252_R, SOX1_P294_F, WT1_E32_F, WNT2_P217_F, MDR1_seq_42_S300_R). The gene discussed is WT1; the disease is neoplasm.