AKT1 and neoplasm: It has been suggested that phosphorylation of the Ser473 may be independent of its activity (Hill et al., 2001) or it may not be necessary for the full activation of Akt (Moore et al., 2011) and that phosphorylation of Thr308 is a more reliable biomarker than that of Ser473 for Akt activity especially in tumor samples (Guertin et al., 2006; Vincent et al., 2011).