In this retrospective study, the protein expressions of Cx43, VEGF, and CD105 in HCC tissue with different serum AFP levels were examined by tissue microarray and immunohistochemical staining; expression of Cx43 mRNA was examined by RT-PCR to explore the relationship between Cx43 and early recurrence, metastasis, and prognosis in patients with HCC after radical resection. This evidence concerns the gene AFP and hepatocellular carcinoma.