As shown in Figure 4A, the EC50 values for GLV-1h189 and GLV-1h285, upon infecting the representative GBM CSC line 040622, were quite different with a substantially larger amount (22.14 fold) of GLV-1h189 required for the same degree of growth inhibition as GLV-1h285, suggesting that BMP-4 production might have a general role in facilitating VACV replication in GBM patient samples. This evidence concerns the gene BMP4 and glioblastoma.