TNF and myalgic encephalomeyelitis/chronic fatigue syndrome: In recent work, female adolescent CFS patients (n = 67 females; age 15 ± 1.4 years) showed a distinct immune profile when compared to acutely fatigued or non-fatigued participants, including increased production of anti-inflammatory cytokines IL-10, decreased IFNγ /IL-10 ratio and reduced production of pro-inflammatory cytokines IL-6, and TNFα in response to lipopolysaccaride (LPS) challenge in vitro[12].