By using a murine Th2-mediated airway inflammation model, we demonstrated that the administration of ATRA attenuated OVA-induced airway inflammation by decreasing Th2 and Th17 related cytokines and inflammatory cells in the airway and ATRA mediated reduction of Th2 cytokines was via inhibiting GATA-3 expression. This evidence concerns the gene GATA3 and inflammatory response.