In order to detect the effects of CXCR4 on invasion of esophageal carcinoma, we used transwell invasion assay and found that KYSE-150 and TE-13 penetrating cells transfected with CXCR4 siRNA1 and CXCR4 siRNA2 reduced significantly compared to negative control and blank control group (Figure 3). Here, CXCR4 is linked to carcinoma of esophagus.