In this study, we suggest that Selaginellatamariscina exhibits beneficial effects on oral cancer cell treatment by (1) inhibiting HSC-3 oral cancer cell migration and invasion, (2) reducing MMP-2 and MMP-9 gene expression and enzyme activity, (3) inhibiting phosphorylation of AKT, (4) decreasing nuclear translocation of CREB and SP-1 to an MMP-2 promoter, and (5) decreasing nuclear translocation of AP-1 to an MMP-9 promoter. Here, SP1 is linked to lip and oral cavity carcinoma.