ADPGK is highly expressed in human tumours and tumour cell lines, at both the mRNA and protein levels, although there is little indication of up-regulation relative to normal tissues and (unlike many glycolytic enzymes) it is not up-regulated by anoxia, hypoxia or HIF-1 in tumour cell cultures and shows little dependence on extracellular glucose concentration [13]. This evidence concerns the gene ADPGK and neoplasm.