Preliminary analysis of brains isolated from mice at day 2 post-infection, labelled with rabbit anti-GFAP 594 (pink) to detect glial fibrillary acidic protein, the main intermediate filament protein expressed by astrocytes, revealed GVR35 eGFP parasites in the cerebellar parenchyma (Figure 2d), suggesting that trypanosome CNS invasion occurs before neurological signs are detected. This evidence concerns the gene GFAP and infection.