These observation were further confirmed by exposing MM cells to 5’ azacytidine treatment that led to increase of GITR levels, both in MM.1S and OPM1 cells (Figure 1d), while no relatively significant increase of GITR expression was obtained by performing qPCR in INA6, U266 and RPMI8226 cell lines, which were characterized by lower methylation pattern of GITR promoter (Figure S1d). The gene discussed is TNFRSF18; the disease is Miyoshi myopathy.