Classically activated or M1 macrophages are activated by ligands of toll-like receptors (TLRs) and pro-inflammatory cytokines (e.g., interferon γ—IFNγ, tumor necrosis factor α—TNFα, interleukin 1β—IL-1β), they activate the Th1 immune response and secrete high amounts of pro-inflammatory mediators that kill the invading pathogens or tumor cells, such as the cytotoxic TNFα and NO. The gene discussed is TNF; the disease is neoplasm.