VIM and breast neoplasm: To characterize more in-depth the implications of vimentin expression in the context of EMT, we expressed GFP-vimentin in both a human melanoma cell line (Figure 5A) and a human breast tumor cell line with an epithelial phenotype (MCF7) (Figure 5B and 5C); MCF7 cells were chosen due to the lack endogenous vimentin expression compared with melanoma G361 cells (Figure 5A, G361 cells and Figure 5B and 5C, MCF7 cells).