IL4R and infectious otitis media: First, in an independent experiment using the ACF protocol, we confirmed our previous finding (17) that the number of colorectal ACFs that developed 6 weeks after AOM administration in BALB/c IL-4Rα−/− mice [median 8.5 ACFs per mouse (interquartile range, IQR 5.75–12), n = 8] was significantly higher than the ACF multiplicity in WT controls [median 3 ACFs per mouse (IQR 1–3.5), n = 9; P = 0.007; Figure 1A].