To elucidate the relative contribution of perfringolysin and alpha toxin in the induction of necrohemorrhagic enteritis a wild-type strain and its ΔpfoA Δplc mutant was used in combination with the ΔpfoA Δplc mutant complemented with either the pfoA or the plc gene or both (Figure 2). The gene discussed is HSPG2; the disease is enteritis.