In this study, we determined that AA is selectively toxic to several non-small cell lung cancer (NSCLC) cell lines and that combination of AA and 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one (3-PO), a novel inhibitor of PFKFB3 with significant anticancer activity [9], synergistically induces apoptosis in NSCLC cells. This evidence concerns the gene PFKFB3 and non-small cell lung carcinoma.