We examine whether aberrant DNA methylation of the PEG1/MEST and IGF2/H19 regulatory regions and of three other imprinted gene DMRs belonging to this imprinted gene cluster: Pleomorphic Adenoma Gene-Like 1 (PLAGL1), Neuronatin (NNAT), Delta-like homolog 1 and noncoding Maternally Expressed Gene 3 (DLK1/MEG3), is associated with CIN and ICC, taking into account HPV infection. The gene discussed is PLAGL1; the disease is intrahepatic cholangiocarcinoma.