ACTB and neoplasm: To further validate the optical resolution that could be achieved with the WBS-PCR, we investigated the biodistribution pattern of human mRNAs encoding ELAVL1 (embryonic lethal, abnormal vision, Drosophila-like 1), Actb (Beta actin) and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) in a section obtained from a human tumour (HCT-116)-bearing mouse (Figure 1c) using human specific RT-qPCR primers.