Based on the fact that hFVIII integrated at the rDNA locus of several human cell lines expressed efficiently [14] and MSCs can home to sites of ongoing injury/inflammation to release FVIII [43], hFVIII-expressing MSCs generated using the method described herein may bring great hope for the autologous therapy of the hemophilia A, which is the most common inheritable deficiency of coagulation. The gene discussed is F8; the disease is hemophilia A.