The top affected pathways (e.g., electron transport chain and glucose activation of insulin secretion), molecular functions (e.g., oxidoreductase activity), biological processes (e.g., oxoacid metabolic process, and cellular catabolic process, cellular ketone metabolic process), and diseases (e.g., Leigh disease, Leigh syndrome) are related to mitochondria further confirming that trans-10, cis-12-CLA feeding affected hepatic mitochondrial functions. Here, INS is linked to Leigh syndrome.