NFATC2 and glioblastoma: In 50 clinical samples (25P and 25A), immunohistochemistry stain showed that NFAT1 protein was overexpressed and mainly located in the nuclei in GBM cells (23/25), while in astrocytomas NFAT1 was expressed at a low level and confined to the cytoplasm (6/25) (Fig. 1C).