TGFB1 and chronic obstructive pulmonary disease: At the 4 h and 8 h time points there were no significant changes in FBLN-1 mRNA levels, however, at 24 h, 48 h, and 72 h time points the FBLN-1 mRNA expression level was significantly down regulated by TGF-β1 both in COPD (p<0.001, n = 7) and non-COPD (p<0.05, p<0.001, n = 5) ASM cells.