Previous studies performed in vitro have shown that NSAIDs such SS and Indomethacin can activate DNA binding activity of HSF-1 in tumor cell lines such as HeLa cells without an increased in HSF1-dependent transcription of HSP-72 at supra-pharmacological concentrations (SS from 2 to 30 mM and Indomethacin from 100 to 1000 μM) [6], [7], [11], [12] our results confirm these effects of ASA on HSF-1 activation without transcription activity. Here, HSF1 is linked to neoplasm.