We additionally analyzed MNNG- and TNF-induced activation/disappearance of PARP-1, depletion of intracellular ATP and loss of membrane integrity in human leukemic Jurkat T cells and human HT-29 colorectal adenocarcinoma cells as two additional established cell systems for necroptosis [6, 17]. The gene discussed is TNF; the disease is colorectal adenocarcinoma.