To examine the expression pattern of PTK6 in NPC, we first performed a western blotting analysis using a monoclonal antibody against PTK6 on 6 NPC cell lines including CNE1, CNE2, 6-10B, 5-8F, HONE1, SUNE1, and HNE1 and two immortalized nasopharyngeal epithelial cells (NPEC1 Bmi-1 and NPEC2 Bmi-1). The gene discussed is BMI1; the disease is nasopharyngeal carcinoma.