In summary, we observed the highly expressed PTK6 in NPC and overexpression of PTK6 contributed increases in cell proliferation and colony formation ability of NPC cells, and demonstrated that high level of PTK6 is closely correlated with poor prognosis of NPC, suggesting that PTK6 may serve as a useful biomarker to determine the prognosis of NPC and provide a potential therapeutical target for NPC treatment. Here, PTK6 is linked to nasopharyngeal carcinoma.