To test whether GRN could aggravate LN by enhancing ALD-DNA-induced M2b-polarized macrophages, we first isolated CD11b+/F4/80high renal macrophages from pGRN or pcDNA- 3.1-treated lupus mice, LV-shGRN or LV-shNC-injected lupus mice. This evidence concerns the gene ITGAM and systemic lupus erythematosus.