In the present study, by using several biophysical methods, such as 90° light scattering, dynamic light scattering, atomic force microscopy (AFM), mass spectrometry (MS), size-exclusion chromatography, and isothermal titration calorimetry (ITC), we investigated and compared the effects of cupric ions on the aggregation of ALS-associated SOD1 mutant A4V and wild-type SOD1 oxidized by hydrogen peroxide. This evidence concerns the gene SOD1 and amyotrophic lateral sclerosis.