As shown in Figure 5A and B, the level of STAT3, JAK-phosphorylated form of STAT3, indicated as pTyr705-STAT3 and PKC-phosphorylated form of STAT3, indicated as pSer727-STAT3, were enhanced in VSMCs cultured in 10% serum from HFD/STZ-induced T2D mice. This evidence concerns the gene STAT3 and type 2 diabetes mellitus.