ANXA5 and breast carcinoma: In order to further investigate the biological effect of miR-26a ectopic restoration on apoptosis in breast cancer cells, we transiently transfected MDA-MB-231 and MCF-7 cells with miR-26a and measured the apoptotic cell death assay using flow cytometric analysis of Annexin V-FITC/PI staining (Fig. 4).