The methylation status of the intron 3 CTCF consensus sequence and correlation with expression of IGF2 in ovarian cancer prompted us to hypothesize that methylation status may modulate binding of CTCF and that this in turn is influencing transcriptional activity of IGF2. Our results suggest that methylation of the single CpG dinucleotide within the IGF2 intron 3 CTCF binding motif may be inhibitory to CTCF binding. The gene discussed is CTCF; the disease is ovarian cancer.