As shown in Fig. 3B, C60 could effectively inhibit infection by both HIV-1 IIIB and Bal with IC50 values of 15.28±4.56 μM and 9.21±3.25 μM, respectively, while it showed no inhibition of infection by either VSV-G or IAV HA pseudoviruses, suggesting that C60 can specifically interact with the HIV-1 Env and inhibit gp41-mediated membrane fusion and, hence, HIV-1 infection. This evidence concerns the gene ERVW-1 and HIV-1 infection.