LTBP4 and esophageal cancer: After demethylation treatment with 5-aza-2’-deoxycytidine LTBP4 expression was induced in OE33 and KYSE180 cells by more than 150% and 60%, respectively (Figure 3A), whereas demethylation treatment with 5-aza-2’-deoxycytidine did not significantly change TGF-β1 expression in both esophageal cancer cell lines (Figure 3B).