In summary, the major novel findings which emerged from this study are that modified expression of Markov Blanket COL4A1, EGFR, BTF3, MPP2, RAB31, CDK4, CD99, ANXA2, TOP2A, and SERBP1 genes are associated with the development of glioblastoma, a highest form of astrocytoma. This evidence concerns the gene CDK4 and astrocytoma (excluding glioblastoma).