The results showed the anti-proliferation, anti-migration and pro-apoptotic effects of UA in colon cancer cells were mediated through simultaneous modulation of multiple signaling pathways, including MMP9/CDH1, Akt/ERK, COX-2/PGE2, p300/NF-κB/CREB2 and cytochrome c/caspase-dependent pathways. The gene discussed is MMP9; the disease is colonic neoplasm.