To address how CNS myeloid cells respond to LCMV in the absence of IFN-I signaling, we imaged the brains of OT-I IFNR−/− CX3CRI-GFP+/− mice by TPLSM at day 15 post-infection – a time point when a robust innate myeloid response is typically observed (Fig. 4). This evidence concerns the gene IFNAR2 and infection.