To ascertain that Beclin-1 mediates Bosutinib effects on amyloid clearance, rat B35 neuroblastoma cells were transfected with 3 μg human cDNA Aβ1–42 (or LacZ) or Beclin-1 shRNA for 24 h, and then treated with 1 μM Bosutinib for an additional 24 h. The gene discussed is BECN1; the disease is neuroblastoma.