Plasmids pEPito-CMV-EGFPLuc, pEPito-AFP-EGFPLuc and pEPito-hCMV/AFP-EGFPLuc were transfected into 13 different human and murine cancer cell lines (hepatoma, cervix carcinoma, colon carcinoma, glioma, prostate carcinoma, melanoma, squamous cell carcinoma, neuroblastoma) and a murine fibroblast cell line (Figure 2). The gene discussed is AFP; the disease is hepatocellular carcinoma.