When challenged with Azoxymethane (AOM), a commonly used carcinogen for colon cancer studies, BubR1−/+ mice showed significantly higher number and larger size of Aberrant Crypt Foci (ACF), colonic precancerous lesions, than did wild-type control; and they developed colon tumors (microadenoma) (Dai et al., 2004). This evidence concerns the gene BUB1B and colonic neoplasm.