TGFB1 and breast cancer: To investigate whether TGF-β1, H2O2, and TLR4 ligand might cooperate to promote invasive migration of non-invasive breast cancer cells, we first cultured non-invasive MCF-7 and T-47D cells in presence of TGF-β1, H2O2, and LPS (a well known TLR4 ligand).