There is no difference in the percentage of both ChAT-positive neurons and Hb9-positive neurons between ALS-iPS-1 and C57-iPS-12 groups (ChAT: 83.5±7.9% vs 85.2±6.8%, P>0.05; Hb9: 24.3±2.7% vs 26.1±3.4%, P>0.05), suggesting that SOD1 mutation did not reduce the potency of motoneuron differentiation compared to the normal iPS cell line. This evidence concerns the gene CHAT and amyotrophic lateral sclerosis.