In the present study, we investigate the role of KLF8 in oral cancer and the effects of KLF8 knockdown via lentivirus mediated siRNA infection in human adenosquamos carcinoma CAL 27 cells.Study Design: We developed a vector-based siRNA expression system that can induce RNAi in CAL 27 oral cancer cells. This evidence concerns the gene KLF8 and lip and oral cavity carcinoma.